Analysis of Env RNA expression in lung and airways

Analysis of Env RNA expression in lung and airways Total RNA was isolated from lung, from trachea and from epithelial tissue scraped from the inside of the nose, by using a Polytron tissue homogenizer (Brinkmann) and Trizol RNA isolation reagent (Invitrogen). Samples were treated with DNase and with reverse transcriptase in the presence of a 3 0 Env primer; they were then subjected to 30 cycles of PCR amplification with primers flanking the intron in ARJenv (Fig. 1). Products were subjected to electrophoresis in agarose gels and were stained with ethidium bromide.